The Two-Headed Monster's Mother is the maternal parent of the Two-Headed Monster. She appears at the end of a sketch in which the Two-Headed Monster spells out the word "Mom" on a brick wall. (EKA: Episode 2640) Only when they've successfully deciphered the message does their mother arrive to show great affection for her sons. Suggesting that polycephaly is not necessarily hereditary, she only has one head.

The puppet used as the monster's mother is the Telly Monster puppet with a large, bushy wig and purple nose.