The James Twins are a pair of cowpokes who appear in a Sesame Street Old West sketch. (EKA: Episode 0428) Similar to the Busby Twins, they both speak in alternating, monosyllabic parts.

At the local saloon, word gets out that the James Twins are about to arrive. The patrons began to panic and decide to do whatever the twins ask of them to avoid any trouble. The Twins arrive and demand some music, but due to their speech pattern, the patrons only hear "mu," and start mooing like cows. When they hear "sic," they aren't sure what they mean. Eventually, they put both parts together and everyone enjoys dancing to the saloon piano.

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