The Barbershop Cactus Quartet are a Barbershop quartet that appear in episode 102 of Muppets Tonight. Clifford has promised them that they'd be performing with Garth Brooks, and Rizzo had scheduled them to do so to the song "Papa Loves Mama." However, Brooks has made his own agenda for the show, and the cacti find themselves arguing with The Longhorn Cheddar Cheeses as to who gets to go on with Brooks and when. Ultimately, neither group has the pleasure of appearing with the country singer, the cactus group never singing a note beyond their brief warm-up backstage. One of the cacti joined Garth and the other Muppets in singing We Shall Be Free.
The cactuses also appeared in the official trailer and poster for The Muppets.