- On episode 213 of The Muppet Show, Giuseppe Wagner is the composer of the opera The Barber of Die Fledermaus, a reference to both The Barber of Seville and Die Fledermaus.
- When the Muppet Babies become Teenage Mutant Ninja Turtles in "Sing a Song of Superheroes", they sing a heroic song set to "Largo al factotum".
- An excerpt from the opera is included in the book and tape set Grover's Overtures.
- The character Signora Barbara F. Seville from the 2001 album Elmo and the Orchestra is a play on the title character's name.
- Placido Flamingo has sung many operas based on The Barber of Seville, including "The Bathtub of Seville", "Dentist of Seville", "The Shirt of Seville", and "The Telephone of Brazil.
- A turkey tries to sing "Largo al factotum" from the opera in a Big Bird and Snuffy bumper on Sesame Street.
- Isabel Leonard sings a portion of the opera with Murray Monster and Ovejita in Sesame Street Episode 4326.
- Bugs Bunny starred in Rabbit of Seville