The film has been spoofed a number of times with the Muppets.
- Grover spoofs John Travolta's famous Saturday Night Fever pose on the cover of the 1978 LP Sesame Street Fever. The inside of the jacket features Grover in more Travolta poses, spoofing the inside of the Saturday Night Fever album.
- In the Sesame Street Live show Elmo Makes Music, Bert takes the stage in John Travolta's white suit and dances to "The Hustle".
- The 1982 Miss Piggy Calendar parody "Saturday Night Greaser" is a spoof of two popular films, Grease and Saturday Night Fever.
- Animal appears dressed as Travolta on a licensed Muppet postcard that parodies the film as "Saturday Night Furball", and similar pictures were used for various merchandise, including magnets.
- Fran Drescher played Connie
- Donna Pescow played Annette
- Robb Sapp played Bobby C. in a stage production of the musical
- John Travolta played Tony Manero