Sam Spayed is a classic animated detective hero who appears in the Dog City episode "Old Dogs, New Tricks." Sam was created as a black and white character by Scratch McCollie, and with an updated design, takes a mentor role towards Ace Hart, as they attempt to stop Baron Von Rottweiler.

His name is a take on Dashiell Hammett's character Sam Spade from The Maltese Falcon, and his voice is an imitation of Humphrey Bogart's from the 1941 film version.