Mr. Tinkles is a pampered Persian cat who takes the principal villain role in Cats & Dogs. Mr. Tinkles takes up residence in the home of wealthy industrialist Mr. Mason, and is spoiled by the cheerful maid, Sophie. Mr. Tinkles plots to use Mason's company to orchestrate his plans for world domination, including the destruction of an experimental cure for allergic reactions to dogs.

Prior to the film debut of Cats & Dogs, Mr. Tinkles made a special appearance at MuppetFest, singing "That's Life."