- The Sesame Street reoccurring segment "Murray Has a Little Lamb", is a play on the famed nursery rhyme, and features a rap variation of the classic song.
- The Mother Goose Stories episode "Mary's Little Lamb" is based on the nursery rhyme.
- In a Sesame Street News Flash segment, Don Music is trying to compose "Mary Had a Little Lamb," but he can't find a rhyme for "snow." Kermit suggests he try a new angle and the result is "Mary Had a Bicycle". And in another News Flash segment, Kermit interviews Mary and her lamb, which follows Kermit home.
- In the News Flash segment "Cookie Red Riding Hood", the doorbell to Red Riding Hood's Grandmother plays "Mary Had a Little Lamb."
- In yet another New Flash segment, Kermit encounters a distressed Little Bo Peep, whose sheep is missing. One of the sheep they interview claims to be Mary's little lamb.
- Honkers attempt to play "Mary Had a Little Lamb" on their noses in episode1576 of Sesame Street.
- Big Bird and the Birdketeers welcome Mary and her little lamb to their nest, but the group decide to change Mary's nursery rhyme to "Jenny Had a Little Jam" for the day.
- In episode 4010 of Sesame Street, Elmo, Zoe, Telly and Rosita perform a reenactment of "Mary Had a Little Lamb."
- A string quartet tunes up before it plays "Mary Had a Little Lamb."(EKA: Episode 3811)
- David sings "Mary Had a Little Lamb" while Linda signs it.(EKA: Episode 2283)
- Shadow tells the story of "Mary Had a Little Lamb" in Friends at Play of Bear in the Big Blue House.
- Special footage was shot for the "Mary Had a Little Lamb" segment of Kids' Favorite Songs 2 video.
- In the book Muppet Babies' Classic Nursery Rhymes, Baby Piggy takes on the role of Mary.
- The interactive video game Let's Learn to Play Together features the song.
- The nursery ryhme is featured in the books The Sesame Street Library Volume 2, The Sesame Street Treasury Volume 15, Read Along with Elmo, Elmo Plays Piano, Big Book of Nursery Rhymes & Fairy Tales, Muppet Treasury,
- Versions of the song are included on the recordings Big Bird Leads the Band,