thumb|300px|"Mary Had a Little Jam"
- The Sesame Street reoccurring segment "Murray Has a Little Lamb", is a play on the famed nursery rhyme, and features a rap variation of the classic song.
- The Mother Goose Stories episode "Mary's Little Lamb" is based on the nursery rhyme.
- In a Sesame Street News Flash segment, Don Music is trying to compose "Mary Had a Little Lamb," but he can't find a rhyme for "snow." Kermit suggests he try a new angle and the result is "Mary Had a Bicycle". And in another News Flash segment, Kermit interviews Mary and her lamb, which follows Kermit home.
- Honkers attempt to play "Mary Had a Little Lamb" on their noses in episode1576 of Sesame Street.
- Big Bird and the Birdketeers welcome Mary and her little lamb to their nest, but the group decide to change Mary's nursery rhyme to "Jenny Had a Little Jam" for the day.
- In episode 4010 of Sesame Street, Elmo, Zoe, Telly and Rosita perform a reenactment of "Mary Had a Little Lamb."
- A string quartet tunes up before it plays "Mary Had a Little Lamb."(EKA: Episode 3811)
- David sings "Mary Had a Little Lamb" while Linda signs it.(EKA: Episode 2283)
- Special footage was shot for the "Mary Had a Little Lamb" segment of Kids' Favorite Songs 2 video.
- In the book Muppet Babies' Classic Nursery Rhymes, Baby Piggy takes on the role of Mary.