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Little Miss Muffet is the titular character in the Little Miss Muffet nursery rhyme. She appeared in a Season 3 Sesame Street sketch, preparing for a theatrical performance of the poem, and becomes increasingly frustrated by her Fat Blue co-star's costume problems. She's also taken aback when director Lance addresses her as "lady." She was performed here by Frank Oz and made out of the Large Lavender Live Hand Anything Muppet.
In later appearances, Miss Muffet has often appeared as a Lavender Anything Muppet. She has gone through many performers; including Jerry Nelson (in an early Sesame Street News Flash), Camille Bonora (in a season 20 episode), Kathryn Mullen (in a retelling of the story read by Gina), Fran Brill (in the video Let's Eat!: Funny Food Songs), and Stephanie D'Abruzzo (in a Season 45 episode).