The puppet has often been used as a background character on Sesame Street during street scenes, sketches, or songs such as, "Boogie Woogie Piggies" and "If I Were The Letter B." Rick Lyon has performed the character in the background.
- Occasionally, the Katheleen puppet would be used for cows with different names. In the "The New Here is Your Life" sketch which focuses on Prairie Dawn's glass of milk, the Katheleen puppet is named Bossy and performed by Camille Bonora. In the "Law and Order: Special Letters Unit" sketch, the cow is named Murray, played by Eric Jacobson, and is considered the missing letter "M" because it makes a moo sound.
- In A Muppet Family Christmas, the Kathleen puppet is performed by Richard Hunt (using a voice similar to Gladys) during "Deck the Halls."