Fallon appeared in two Sesame Street episodes in 2009, during the 40th season. In episode 4190, Fallon played "Wild Nature Survivor Guy", trying to survive alone in the wild even though he's in the middle of the city with a bunch of people. Fallon also appeared in episode 4205, learning about the word "inspect" with Elmo.
On the March 4, 2009 episode of his talk show, Fallon talked with Cameron Diaz about their visits to the Sesame Street set. Diaz mentioned that everyone on the set loved Fallon, while Fallon expressed his admiration for the puppeteers.
On June 8, 2010, he appeared on Today alongside Frank Oz and Meredith Vieira to unveil the Muppet Pipes as part of the NBC studio tour. Fallon had petitioned NBC executives to put the pipes behind glass and add them to the tour when he took over Late Night from Conan O'Brien, as they had previously been hidden in a closet in Max Weinberg's dressing room.
Elmo is to appear on the show May 3.