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ER is a television series about a group of emergency room doctors working at a Chicago hospital. The show has ran on NBC from 1994 until 2009. George Clooney's character, Dr. Doug Ross was ranked #95 the television special 100 Greatest TV Characters.
- Muppets Tonight’s E-I-E-I-OR is a spoof of the series.
- Noah Wyle and Julianna Margulies had guest spots as friendly doctors on Sesame Street. Wyle's guest spot was sponsored by the letters "E and R."
- In episode 3910 of Sesame Street, the monsters have signs with different parts of the word "COOPERATE." Lulu's portion reads "ER," which she believes is the name of a television show.
- The title for a second season episode, "It's Not Easy Being Greene" is a reference to the song Bein' Green.
- Alan Alda played Dr. Gabriel Lawrence
- Tom Arnold played The Big Kahuna in "I Feel Good" (2009)
- Ed Asner played a clinician
- John Aylward plays Dr. Anspaugh
- Red Buttons played Jules 'Ruby' Rubadoux
- Rosemary Clooney played Madame X
- James Cromwell played a Roman Catholic Bishop
- Alexis Cruz played Terry
- Paul Dooley played Henry Lewis
- Dakota Fanning played Delia Chadsey in ("The Fastest Year," 2000)
- Conchata Ferrell played Mrs. Jenkins
- Sally Field played Maggie Wyczenski
- Mariska Hargitay played Cynthia Hooper
- Jonathan Morgan Heit played Jessie ("Dying Is Easy," 2007)
- Danny Glover played Charlie Pratt Sr.
- Mimi Kennedy played Sergeant O'Malley ("Officer Down", 2007)
- Harvey Korman played Stan Levy ("Stuck on You", 1998)
- Joanna Krupa appeared in the episode "Hit and Run" (1994)
- John Leguizamo played Dr. Victor Clemente
- Ray Liotta played Charlie Metcalf
- William H. Macy played Dr. David Morgenstern
- Brandon Maggart played Stan Calaus ("A Miracle Happens Here", 1995)
- Mary Mara played Loretta Sweet
- Julianna Margulies played Carol Hathaway
- Chris Marquette played Marty Dorset in "Abby Road" (2000)
- Kellie Martin played Lucy Knight
- Whitman Mayo played Jesse Morgan in "Middle of Nowhere" (1999)
- Bob Newhart played Ben Hollander
- Kim Raver played Paramedic Kim Zambrano (her role from 'Third Watch') in "Brothers and Sisters" (2002)
- Lea Salonga played Amparo in "I'll Be Home for Christmas" (2001)
- Allison Smith played Denny in "The Greater Good" (2003)
- Susan Sarandon appeared in "Old Times" (2009)
- David L. Smyrl played Marty on "Brothers and Sisters" (2002)
- Steven Spielberg was excecutive producer in the first season.
- Lisa Waltz played Mrs. Wimbur in "It's Not Easy Being Greene" (1996)
- Gedde Watanabe played Nurse Yosh Takata
- Ellis E. Williams played Bill McClintock in "Feb 5, '95" (1995)
- Forest Whitaker played Curtis Ames
- Noah Wyle played Medical Student (later Dr.) John Carter