There have been a variety of Muppet cows in various productions, including Sesame Street and The Muppet Show. One of the most prominent cows is Gladys the Cow. Other cows on Sesame Street included Natalie (a black and white cow), and a dark brown/red cow.

Occasionally, the Muppets mistake horses for cows. Such confusion occurs during the "Camelot" segment from The Muppet Show episode 305, "A Four-Legged Friend" (where Jim and Jerry ride cows but refer to them as horses), and the western sketch in The Muppets Go to the Movies.

The Muppet Show

• Episode 121: Twiggy
• Episode 211: Dom DeLuise
• Episode 224: Cloris Leachman
• Episode 322: Roy Rogers & Dale Evans - several cows
• Episode 408: Arlo Guthrie - six different cows and a bull.
• Episode 418: Christopher Reeve
• Episode 512: Melissa Manchester - a cow falls on The Newsman

Sesame Street

• Gladys the Cow
• Kathleen the Cow
• Mariah Cowey
• Bossy the Cow
• Natalie, the cow who jumped over the moon in Sesame Street News Flash
• Lola
• Back-up singers for How Now Brown and the Moo Wave

Elsewhere

• Sesamstrasse - Delia Denver, Amelia Kuhhart
• The Muppets on Puppets - Gretel
• National Wildlife Federation - Bessie
• Episode 102: Space Cowboys - Bessie and others
• Muppets Tonight - The Cow Announcer, Lash Holstein, Dorothy Bovine, Moo-ing the Merciless, Moo-ing's Henchman
• Muppets from Space
• Mopatop's Shop - Billy the Bull
• Pajanimals - CowBella
• Dinosaurs - Moola the Cash Cow

In addition to Muppet cows, a real cow appeared backstage in episode 217, and a number of real cows appear in Jim Frawley's Camera Tests for The Muppet Movie.